Weselek, Grit; Keiner, Silke; Fauser, Mareike; Wagenführ, Lisa; Müller, Julia; Kaltschmidt, Barbara; Brandt, Moritz D.; Gerlach, Manfred; Redecker, Christoph; Hermann, Andreas; Storch, Alexander: Norepinephrine is a negative regulator of the adult periventricular neural stem cell niche. In: Stem Cells. Jg.38 H. 9. 2020, S. 1188-1201
Inhalt
- 2 METHODS
- 2.1 Animals
- 2.2 HPLC assay of tissue norepinephrine content
- 2.3 Intracerebroventricular infusion
- 2.4 Neurotoxic lesion with Dsp4
- 2.5 Treatment with thymidine analogs
- 2.6 Tissue processing and immunohistochemistry
- 2.7 Quantitative real-time PCR
- 2.8 Tissue processing for in situ hybridization
- 2.9 PCR-derived antisense-RNA probes
- 2.10 In vitro transcription of RNA probes
- 2.11 In situ hybridization
- 2.12 Adrenoceptor antagonist and norepinephrine treatment in vitro
- 2.13 Immunocytochemistry
- 2.14 Cell counts and statistical analysis
- 3 RESULTS
- 3.1 Norepinephrine innervation of the adult neural stem cell niches negatively correlates with neuroprogenitor cell prolif...
- 3.2 Norepinephrine reversely inhibits SVZ cell proliferation and reduces olfactory bulb neurogenesis
- 3.3 Blocking norepinephrine actions activates adult hypothalamic and midbrain neuroprogenitor proliferation and early neur...
- 3.4 Impairment of norepinephrine innervation decreases adult hippocampal neurogenesis
- 3.5 Norepinephrine suppresses neuroprogenitor proliferation and promotes neurogenesis through β-adrenoceptor signaling
- 4 DISCUSSION
- 5 CONCLUSION
- ACKNOWLEDGMENTS
- CONFLICT OF INTEREST
- AUTHOR CONTRIBUTIONS
- REFERENCES