Höving, Anna Lena; Schmidt, Kazuko E.; Merten, Madlen; Hamidi, Jassin; Rott, Ann-Katrin; Faust, Isabel; Greiner, Johannes; Gummert, Jan; Kaltschmidt, Barbara; Kaltschmidt, Christian; Knabbe, Cornelius: Blood Serum Stimulates p38-Mediated Proliferation and Changes in Global Gene Expression of Adult Human Cardiac Stem Cells. In: Cells. Jg.9 H. 6. 2020
Inhalt
- Materials and Methods
- Isolation and Cultivation of Adult Human Cardiac Stem Cells from Heart Auricles
- Immunohistochemistry and Immunocytochemistry
- Flow Cytometry
- Cardiac Differentiation of hCSCs
- Blood Plasma
- p38-MAPK Inhibition
- Proliferation Assay
- Orangu Cell Viability Assay
- Senescence-Associated -Galactosidase Assay
- RNA Isolation and Sequencing
- ELISA
- Results
- Identification of Nestin+/S100B+ Cells in the Human Heart Auricle Tissue
- Successfully iIsolated Putative Human Cardiac Stem Cells from Heart Auricle Tissue Show High Clonal Efficiency and Capability for Cardiosphere Formation
- Isolated Cells Express Known Marker Proteins of Cardiac Stem and Progenitor Cells
- HCSCs Are Able to Give Rise to Cardiomyocytes In Vitro
- Blood Plasma and Blood Serum Strongly Enhance Proliferation-Inducing Effects of Blood Plasma on Adult Human Cardiac Stem Cells
- Age and Sex of Blood Serum Donors Do Not Affect Beneficial Effects on Proliferation and Metabolism of hCSCs
- Exposure of hCSCs to Blood Serum from Young Female or Male Donors Results in Significantly Enhanced Protection against Senescence Compared to Serum from Old Female Individuals
- Young Blood Serum Enhances Differential Global Gene Expression of hCSCs
- Global Gene Expression Profiling Indicates Age-Dependent Clusters of Blood-Serum-Treated Adult Stem Cells
- Gene Ontology (GO) Term Analysis Reveals Downregulation of Attachment-Associated Genes and Upregulation of Proliferation-Associated GO Terms, Including p38-MAPK
- Beneficial Effects of Blood Plasma on Proliferation and Protection of hCSCs Against Senescence Are Partially Mediated by p38 MAPK-Signaling
- Discussion
- References