Voitsekhovskaia, Irina; Paulus, Constanze; Dahlem, Charlotte; Rebets, Yuriy; Nadmid, Suvd; Zapp, Josef; Axenov-Gribanov, Denis; Rückert, Christian; Timofeyev, Maxim; Kalinowski, Jörn; Kiemer, Alexandra K; Luzhetskyy, Andriy: Baikalomycins A-C, New Aquayamycin-Type Angucyclines Isolated from Lake Baikal Derived Streptomyces sp. IB201691-2A. In: Microorganisms. Jg.8 H. 5. 2020
Inhalt
- Introduction
- Materials and Methods
- Bacterial Strains, Culture Conditions and Routine Procedures
- Sampling and Actinobacteria Isolation
- 16S rRNA Gene Sequencing and Phylogenetic Analysis
- Screening the Culture Conditions for Biological Activity of Streptomyces sp. IB2016I91-2A
- LC-MS and LC-HRMS Analysis
- Isolation and Purification of Compounds 1–5
- Genome Sequencing and Bioinformatics
- Gene Disruption of the Glycosyltransferase Genes baiGT2 and baiGT3
- Cloning of the bai Gene Cluster Using Transformation-Associated Recombination (TAR) Technique
- Biological Activity Assays
- Anticancer Activities of Isolated Compounds
- Results and Discussion
- Isolation and Characterization of Streptomyces sp. IB201691-2A
- Production and Isolation of Baikalomycins
- Structure Elucidation of Baikalomycins
- Biological Activities of Baikalomycins
- Streptomyces sp. IB201691-2A Genome Sequencing and Analysis
- Identification of Baikalomycins Biosynthetic Gene Cluster
- Genes Putatively Involved in Biosynthesis of Aglycone Core
- Genes Putatively Involved in Deoxysugars Biosynthesis and Attachment
- Genes Involved in Regulation, Resistance, and with Unknown Functions
- Inactivation of Genes Encoding Glycosyltransferases in bai Cluster
- References