Choi, Ji-Eun; Shinoda, Suguru; Asano, Yasuhisa; Gröger, Harald: Aldoxime Dehydratase Mutants as Improved Biocatalysts for a Sustainable Synthesis of Biorenewables-Based 2-Furonitrile. In: Catalysts. Jg.10 H. 4. 2020
Inhalt
- Results and Discussion
- High Throughput Screening of OxdYH3-3 Mutants by Using a UV Microspectrometer
- The Improved Activity Measurement of OxdYH3-3 Mutants
- Homology Modelling of OxdYH WT and N266S
- Materials and Methods
- Materials and Preparation of OxdYH3-3 Transformants
- Synthesis of 2-Furfuryl Aldoxime
- Construction of Mutant Library by Error-Prone PCR
- Screening of OxdYH3-3 WT and Mutants by of UV Spectrum Scan
- Analysis of the DNA Sequence of OxdYH3-3 Mutants
- Protein Purification
- Characterization of Purified OxdYH3-3 Catalyst
- Conversion of Aldoximes to Corresponding Nitriles by Using OxdYH3-3 WT and Mutants
- Determination of Conversion from Aldoximes to Nitrile by GC
- Measurement of the UV Spectrum of Aldoximes and Nitriles After the Reaction
- Homology Modelling
- Conclusions
- References