Kaltschmidt, Bernhard; Ennen, Inga; Greiner, Johannes; Dietsch, Robin; Patel, Anant; Kaltschmidt, Barbara; Kaltschmidt, Christian; Hütten, Andreas: Preparation of Terpenoid-Invasomes with Selective Activity against S. aureus and Characterization by Cryo Transmission Electron Microscopy. In: Biomedicines. Jg.8 H. 5. 2020
Inhalt
- Materials and Methods
- Preparation of Invasomes and Encapsulation of Terpeonoids
- Cryo Transmission Electron Microscopy (Cryo TEM)
- Determination of Zeta Potentials
- Evaluation of Invasome Size and Bilayer Thickness
- Growth-Inhibition Zone Assay
- Analysis of Encapsulation Efficiency and Loading Capacity
- Flow Cytometric Measurement of Cell Death
- Statistical Analysis
- Results and Discussion
- Succsessfull Preparation of Invasomes Encapsulating the Terpenoids Thymol, Menthol, Camphor and Cineol
- Encapsulation of Terpenoids Significantly Changes Lamellarity and Size of Invasomes without Affecting Bilayer Thickness
- Invasomes Encapsulating Thymol, Camphor and Cineol Show Bactericidal Activity against S. aureus in a Growth-Inhibition Zone Assay
- Flow Cytometric Analysis of Cell Death Validate High Bactericidal Activity of Thymol-Loaded Invasomes against Gram-Positive S. aureus
- Thymol-Loaded Invasomes Do Not Affect Survival of Gram-Negative E. coli
- Growth-Inhibition Zone Assay Shows Strong Bactericidal Activity of Cineol Invasomes against E. coli
- References