Berckx, Fede; Wibberg, Daniel; Kalinowski, Jörn; Pawlowski, Katharina: The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type.. In: Genes. Jg.11 H. 4. 2020
Inhalt
- Materials and Methods
- Biological Material
- Gene Expression Analysis using Real-Time Quantitative PCR (RT-qPCR)
- Phylogenetic and Synteny Analysis
- Sequence Similarity Analysis
- Tetranucleotide Frequency
- Protein Modelling
- Results
- Synteny Analysis Indicates Differences in Gene Organization of murC in the Four Frankia Clusters
- Consensus Amino Acid Sequences of Frankia MurC2 Shows Higher Similarity to MurC from Plants than from other Actinobacteria
- No Sequence Evidence for Horizontal Gene Transfer (HGT) could be Found
- Protein Modelling Reveals Little Structural Difference among Different Groups of MurC Sequences
- Gene Expression Data Shows Differential Expression of murC1 and murC2 in Frankia Cluster-2 in Nodules, but not in Frankia Cluster-3
- Discussion
- Conclusions
- References