Wang, Tung-Cheng: Application of fluorescence light microscopy with structured illumination techniques to cell inflammatory responses. 2020
Inhalt
- Theory of optical microscopy
- Image formation and resolution limit
- Fluorescence and labeling
- Fluorescence microscopic techniques
- Wide-field microscopy and illumination schemes
- Deconvolution microscopy
- Confocal laser scanning microscopy
- Superresolution microscopy
- Fluorescence microscopy of bacterial endotoxins
- Introduction of endotoxins
- Molecular structure of bacterial endotoxins
- Formation of TLR4 receptor complex
- Signaling transduction of endotoxins
- Fluorescence imaging of LPS
- Deconvolution microscopy of internalized LPS
- Structured illumination microscopy of endosomal LPS
- Single-molecule tracking of membrane-bound LPS
- Discussion and outlook
- Fluorescence imaging of LOS
- Synthesis of Conjugatable CDE-Cy7N.
- Bioconjugation of CDE-Cy7N to LOS.
- Stoichiometry of CDE-Cy7N to LOS.
- Photophysical property of LOS-Cy7N
- Evaluation of LOS-Cy7N Bioactivity
- Endocytosis of Cy7N-Labeled LOS.
- Discussion and outlook
- Fluorescence imaging of lipid A
- Superresolution microscopy of inflammasome formation
- Introduction of inflammasomes
- Superresolution of fixed ASC specks
- Localization of sensor and effector in specks
- Living speck imaging by 4D-SIM
- Structural analysis of the ASC specks
- Discussion and outlook
- Other SR-SIM projects
- Conclusion
- Bibliography
- Materials and methods
- Publication
- Acknowledgment