Bergmann, Stephan: Optimizing single molecule localization microscopy as a tool in chemistry, biology, and physics. 2020
Inhalt
- Introduction
- Theoretical background
- Widefield microscopy
- Luminescence of fluorescent molecules, nanodiamonds and semiconductor particles
- General widefield fluorescence setup
- Illumination schemes
- Resolution limit in microscopy
- Photochemistry and photophysics of organic fluorophores and fluorescent proteins in specialized buffer
- Approaches to super-resolution microscopy
- Single Molecule Localization Microscopy
- Precise and accurate localization of single emitters in SMLM
- Methods for resolution estimation in SMLM
- 3D single molecule localization microscopy
- Microgels: polymer based colloids with smart properties
- SMLM resolves Network Morphology of Smart Colloidal Microgels
- Introduction
- dSTORM
- Photon correlation spectroscopy
- Fluorescence correlation spectroscopy
- Atomic force microscopy
- Results and discussion
- Conclusion
- Influence of the monomer structure on the particle morphology
- Introduction
- Experimental section
- Microscope setup
- 3D calibration for dSTORM imaging
- Localization density calculation for morphology estimations
- Results and discussion
- Conclusion
- Resolving the internal morphology of core-shell microgels with SMLM
- Introduction
- Experimental Section
- Computational Methods
- 3D quantification of 2D projected single molecule localization microscopy data
- Normalized integrated intensity profile for 3D recalculation of 2D SIM data
- Results and Discussion
- Conclusion
- PALM of cardiomyopathy associated plakophilin-2 mutants
- Probing magnetic fields with super-resolved fluorescence microscopy
- Introduction
- Experimental section
- Fluorescence spectroscopy
- Single molecule localization microscopy
- Evaluation of single molecule localization data
- Results and discussion
- Chiral induced spin selectivity effect
- Summary and comprehensive outlook
- Appendix
- SMLM resolves Network Morphology of Smart Colloidal Microgels
- [Influence of the monomer structure on the particle morphology
- Resolving the internal morphology of core-shell microgels with SMLM
- PALM of cardiomyopathy associated plakophilin-2 mutants
- Probing magnetic fields via super-resolved fluorescence microscopy
- Sample preparation of single molecule surfaces investigating MFE on standard organic fluorophores
- Sample preparation of SAMs on softferromagnetic multilayer systems
- dSTORM imaging buffer
- Acronyms
- Publications
- Acknowledgment
- Statutory declaration