Sandmeyer, Alice: Compact realizations of optical super-resolution microscopy for the life sciences. 2019
Inhalt
- Introduction and motivation
- Theoretical background
- Basics of fluorescence
- Conventional microscopy
- Definitions of resolution limits
- Resolution determination concepts
- Wide-field microscopy
- Deconvolution methods
- Super-resolution microscopy
- Stimulated emission depletion microscopy
- Single molecule localization microscopy
- Super-resolution optical fluctuation imaging
- Structured illumination microscopy
- Fundamentals of biotechnology
- Live-cell imaging of HIV-1 particle dynamics at the virological synapse with a compact 3D fluorescence microscope
- Introduction
- Fluorescence wide-field microscope for 3D deconvolution
- Construction of the optical setup
- Communication flowchart between the devices
- Data acquisition and image processing
- Imaging HIV-1 particles at the virological synapse
- Infection pathway and virological synapse
- Labeling and imaging strategies
- Visualizing the dynamics of the HIV-1 transfer at the VS
- Using 3D-SIM to resolve the transfer of HIV-1
- Discussion and conclusion
- Cost-efficient solution for SOFI with direct camera comparison
- Introduction
- Direct comparison of camera sensors on a scientific microscope
- Microscope setting and data acquisition
- Metric comparison of SOFI reconstructions from the different camera chips
- Setting up cost-effective SOFI on a home-built system
- Discussion and conclusion
- DMD-based SIM visualizes live cell dynamics and reveals morphology of microgels
- Introduction
- Analysis of blazed grating effect
- Coherent illumination of DMDs
- Effect of underlying blazed grating
- Simulation of the blazed grating effect
- Comparison of simulations and experimental results
- Construction of a compact and cost-efficient SIM system
- SIM images of biological samples
- SIM revealing the morphology of microgels
- Discussion and conclusion
- Comprehensive conclusion and outlook
- Bibliography
- Materials and methods
- Acronyms
- Publications
- Acknowledgments
- Statutory declaration