Ruwe, Matthias; Persicke, Marcus; Busche, Tobias; Müller, Benjamin; Kalinowski, Jörn: Physiology and Transcriptional Analysis of (p)ppGpp-Related Regulatory Effects in Corynebacterium glutamicum. In: Frontiers in Microbiology. Jg.10. 2019
Inhalt
- Materials and Methods
- Bacterial Strains and Growth Condition
- Deletion of crtR in C. glutamicum Strains
- 32P-Based Nucleotide Measurements
- Transcriptome Measurements
- Decaprenoxanthin Measurements
- Amino Acid Measurements
- Vitamin Measurements
- Metabolite Measurements
- Whole Genome Sequencing of Cultivation Replicates
- Results
- Total Starvation, Antibiotic Stress and Iron Stress Lead to a Significant Accumulation of Alarmones in C. glutamicum and a Nucleotide Spectrum Differing From That of E. coli
- The Bifunctional RSH Protein RelCg Is the Key Player Enzyme of Total Starvation Induced ppGpp Production
- A C. glutamicum (p)ppGpp0 Mutant Exhibits Altered Transcript Amounts of Genes Involved in Sulfur Metabolism-, Amino Acids- and Carotenoid Synthesis
- The (p)ppGpp0 Strain Exhibits Significant Metabolic Changes in Vitamin, Amino Acid and Carotenoid Biosynthesis and Shows Unstable Growth Behavior Under the Influence of Light
- Differential Transcriptome Analysis Under Total Starvation Conditions Clarifies Global Effects of (p)ppGpp on Central Metabolism Under Conditions of Nutrient Deficiency*-1pt
- Promotor Sequence Analysis of Genes Exclusively Regulated in Strain CR099 Reveals DNA Signatures Associated With ppGpp-Dependent Transcriptional Regulation
- Discussion*1pt
- Stress Associated Alarmone Production in C. glutamicum: In Search of Magic Spot Three*2pt
- Basal Level Effects of Alarmones in C. glutamicum: (p)ppGpp Probably Work as a Fine Tuner of Central Metabolism Processes
- (p)ppGpp-Dependent Transcriptional Regulation in C. glutamicum: Evidence of a Complex Interplay Involving Sigma Factors and Transcriptional Regulators
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References