Moritzer, Ann-Christin; Niemann, Hartmut: Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.. In: Protein science. Jg.28 H. 12. 2019, S. 2112-2118
Inhalt
- 2.1 Structure determination
- 2.2 Binding of FAD to Thal results in closing of the FAD loop
- 2.3 Simultaneous soaking of FAD and l-Trp impairs the binding of cofactor and substrate
- 3 DISCUSSION
- 4 MATERIALS AND METHODS
- 4.1 Protein expression, purification, and crystallization
- 4.2 Data collection, structure determination, and refinement
- ACKNOWLEDGMENTS
- CONFLICT OF INTEREST
- REFERENCES