Markwirth, Andreas; Lachetta, Mario; Mönkemöller, Viola; Heintzmann, Rainer; Hübner, Wolfgang; Huser, Thomas; Müller, Marcel: Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. In: Nature Communications. Jg.10 H. 1. 2019
Inhalt
- Video-rate acquisition and immediate reconstruction
- Discussion
- Methods
- Microscope system concept
- Laser light sources
- Multicolor achromatic optical path
- SLM pattern generation and filtering
- Timing of SLM and camera
- Network transfer and reconstruction pipeline
- GPU-assisted SIM reconstruction
- System control software
- Limitations
- Calibration samples
- Diffusing microsphere sample preparation
- U2OS sample preparation
- 0.04 µm FluoSpheres sample preparation
- Reporting summary
- Data availability
- Code availability
- References
- Acknowledgements
- Author contributions
- Additional information