Gil Lopez, Marina; Irla, Marta; Fernandes de Brito, Luciana; Wendisch, Volker F.: Characterization of D-arabitol as newly discovered carbon source of Bacillus methanolicus. In: Frontiers in Microbiology. Jg.10. 2019
Inhalt
- Materials and Methods
- Bacterial Strains, Media and Cultivation Conditions
- Recombinant DNA Work
- Heterologous Expression of B. methanolicus Genes in C. glutamicum
- Isolation of Total RNA
- cDNA Library Preparation, RNA-Seq and Mapping of Generated RNA-Seq Data
- Real-Time Quantitative Reverse Transcription PCR (qRT-PCR)
- Reverse Transcription PCR (RT-PCR) Analysis of Operon Structure
- Arabitol Phosphate Dehydrogenase Enzymatic Assay of B. methanolicus Crude Extracts
- Analysis of Culture Supernatants by Liquid Chromatography
- Results
- Growth of B. methanolicus MGA3 on Arabitol as Single Carbon Source and as Co-substrate to Mannitol
- Comparative Analysis of Global Gene Expression Profiles of B. methanolicus MGA3 During Growth With Arabitol or Mannitol
- Complementation of the Arabitol-Negative C. glutamicum Mutant ΔmtlD by Heterologous Expression of B. methanolicus MGA3 atlABCD or atlD
- Transcriptional Organization of the Arabitol Operon
- Arabitol Phosphate Dehydrogenase Activity of B. methanolicus MGA3 Crude Extracts
- Discussion
- Data Availability
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References