Verwaaijen, Bart; Wibberg, Daniel; Winkler, Anika; Zrenner, Rita; Bednarz, Hanna; Niehaus, Karsten; Grosch, Rita; Pühler, Alfred; Schlüter, Andreas: A comprehensive analysis of the Lactuca sativa, L. transcriptome during different stages of the compatible interaction with Rhizoctonia solani. In: Scientific Reports. Jg.9 H. 1. 2019
Inhalt
- Experimental setup.
- Mapping of RNA-Seq reads.
- Transcription analysis.
- Functional enrichment of expressed genes.
- Scanning Electron Microscopy.
- Metabolome analysis.
- Figure 1 (A) Exemplary representation of the previously published lettuce leaf interaction model that was used in the current study12.
- Results
- Lettuce/Rhizoctonia solani leaf interaction model.
- Differential expression of lettuce genes during interaction with R. solani.
- Figure 2 Significantly differentially expressed genes (DEGs) among the depicted pairwise comparisons as generated with DESeq using zone 4 (blank) as reference.
- Figure 3 GO term enrichment of lettuce DEGs from interaction zone 1 and 2.
- Differential expression of PRG candidates and WRKY transcription factors.
- The lettuce/R. solani AG1-IB interaction zones show significant shifts in metabolite compositions.
- Figure 4 Mapman analysis between the symptomless interaction zone 1 and zone 2 with light discoloration.
- Discussion
- Reduced transcription of pentatricopeptide genes in zone 1 and 2.
- Reduction of transcripts related to the chloroplast in zone 1 and 2.
- Figure 5 (A) Differential expression of pathogen receptor gene (PRG) candidates.
- Figure 6 Abundances of selected metabolites within the interaction zones.
- Co-transcription of PRG candidates between the interaction zones.
- Comparison of GO term enrichment between lettuce responses to the pathogens R. solani and Botrytis cinerea.
- Transcription of SA and JA signalling genes.
- WRKY transcription factors.
- Metabolite profiles are distinctive for each interaction zone.
- Concluding remarks.
- Acknowledgements