Lämmermann, Nina; Schmid-Michels, Fabian; Weissmann, Aike; Wobbe, Lutz; Hütten, Andreas; Kruse, Olaf: Extremely robust photocurrent generation of titanium dioxide photoanodes bio-sensitized with recombinant microalgal light-harvesting proteins.. In: Scientific reports. Jg.9 H. 1. 2019
Inhalt
- Preparation of functional PSII-associated light-harvesting protein LHCBM6 by in vitro reconstitution.
- Continuous illumination of LHCII-based DSSCs leads to a photoactivation and photocurrent enhancement.
- DSSC deactivation is unrelated to a reduced dye performance.
- Figure 1 In vitro reconstitution of 6xHis-LHCBM6 from inclusion bodies after heterologous expression in E.
- Figure 2 Spectroscopic analysis of sucrose gradient fractions containing refolded His-LHCBM6.
- Figure 3 Composition of the DSSC used in the present work and fluorescence emission spectra of LHCII proteins physisorbed onto the photoanode.
- Figure 4 Correlation between illumination time and DSSC performance determined for two distinct electrolytes (A) I–V curves of LHCII-sensitized TiO2 solar cells in conjunction with an Iodolyte AN-50 (Solaronix) electrolyte (E1).
- Figure 5 DSSC performance under illumination with light lacking the UV part.
- Figure 6 LHCII-sensitized TiO2 photoanodes maintain a high photocurrent during prolonged illumination times.
- Methods
- Fabrication of DSSCs.
- Scanning electron microscopy.
- Characterization of DSSCs.
- Preparation Lhcbm6 apoprotein.
- Pigment isolation and reconstitution of LHCBM6-pigment complexes.
- Purification of reconstituted Lhcbm6.
- Spectroscopy.
- Coating of LHC on electrodes.
- Acknowledgements