Perez, Fernando; Fernandes de Brito, Luciana; Wendisch, Volker F.: Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions. In: Frontiers in Microbiology. Jg.10. 2019, S. 340 ff.
Inhalt
- Strain, Plasmids, and Culture Conditions
- Molecular Biology Methods
- Internal Amino Acids Extraction
- Determination of L-PA by High Pressure Liquid Chromatography
- RNAseq Analysis
- Real-Time Quantitative Reverse Transcription-PCR
- Results
- C. glutamicum Can Use L-PA for Osmoprotection
- The Mechanosensitive Channel YggB May Play a Role as Escape Valve for L-PA
- Comparative RNAseq Analysis of a L-PA Producing C. glutamicum Strain, a L-Proline Producing Strain and the Respective Control Strains
- Role of the Carrier ProP During Growth With L-PA as Osmo Compatible Solute
- Discussion
- Data Availability
- Author Contributions
- Funding
- Acknowledgments
- References