Mindt, Melanie; Walter, Tatjana; Risse, Joe Max; Wendisch, Volker F.: Fermentative production of N-methylglutamate from glycerol by recombinant Pseudomonas putida. In: Frontiers in Bioengineering and Biotechnology. Jg.6. 2018
Inhalt
- Bacterial Strains and Growth Conditions
- Molecular Biological Techniques
- Chromosomal Gene Replacement
- Crude Extract Preparation and Protein Analysis
- Culture Conditions
- Fed-Batch Cultivation of P. putida KT2440 Strains
- Analytical Quantification of Amino Acids and Carbohydrates
- Results
- Establishing Fermentative Production of NMeGlu in P. putida
- Metabolic Engineering for Glycerol-Based NMeGlu Production
- Fed-Batch Mode Production of NMeGlu From Glycerol in Bioreactors
- Discussion
- Author Contributions
- Acknowledgments
- Supplementary Material
- References