Strigli, Anne; Raab, Christian; Hessler, Sabine; Huth, Tobias; Schuldt, Adam J. T.; Alzheimer, Christian; Friedrich, Thomas; Burridge, Paul W.; Luedde, Mark; Schwake, Michael: Doxorubicin induces caspase-mediated proteolysis of KV7.1. In: Communications Biology. Jg.1 H. 1. 2018
Inhalt
- Proteolysis of Kv7.1 produces C-terminal fragments
- Kv7.1 is cleaved by caspases upon apoptosis
- Functional impact of proteolysis on Kv7.1/KCNE1 channels
- Identifying long-QT mutations modulating Kv7.1 proteolysis
- Doxorubicin induces Kv7.1 proteolysis in cardiomyocytes
- Discussion
- Methods
- Plasmids and antibodies
- Cell culture, transfection, and inhibitors
- Protein extraction, immunoprecipitation, and immunoblotting
- Biotinylating assay
- Isolation of neonatal rat ventricular cardiomyocytes
- Human-induced pluripotent stem cell culture
- Cardiac differentiation from human iPS cells
- Electrophysiology
- Statistics
- References
- References
- Acknowledgements
- Author contributions
- Competing interests
- ACKNOWLEDGEMENTS