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Inhalt
- Genome assembly and annotation.
- Fig. 1 Genome size evolution in Salviniales.
- Insights into gene family evolution in land plants.
- The history of whole-genome duplication in ferns.
- The pentatricopeptide repeat family and RNA editing.
- Origin and evolution of a fern insecticidal protein.
- Fig. 2 Evolution of ethylene biosynthesis.
- Fig. 3 The history of WGD in Azolla and Salvinia.
- Fig. 4 Origin of a fern insecticidal protein.
- Methods
- Fig. 5 Azolla–cyanobacterial symbiosis.
- Genome and transcriptome sequencing
- Genome assembly
- Repeat annotation
- Gene prediction
- Phylogenomic inference and placement of WGDs from nuclear gene trees
- Classification of syntenic duplicates and microsynteny analysis
- Gaussian mixture model analysis of Ks distributions
- Gene family classification and ancestral reconstruction
- Transcription-associated protein characterization
- PPR annotation
- Phylogeny of the insecticidal protein Tma12
- Azolla phylogeny
- Cyanobiont phylogeny
- Identification of the CSP genes
- Quantitative real-time PCR of nifH
- Azolla symbiosis transcriptome analysis
- Azolla–cyanobacteria HGT
- Phylogeny of SHC
- Detection of SHC-synthesized triterpenes
- Reporting Summary
- Data availability
- Acknowledgements