Mindt, Melanie; Risse, Joe Max; Gruß, Hendrik; Sewald, Norbert; Eikmanns, Bernhard J.; Wendisch, Volker F.: One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst. In: Scientific Reports. Jg.8 H. 1. 2018
Inhalt
- Results
- Corynebacterium glutamicum as suitable host for NMeAla production.
- Metabolic engineering of C. glutamicum for fermentative production of NMeAla.
- Figure 2 Growth rates of C.
- Figure 3 NMeAla, l-alanine, l-valine and pyruvate production data (A) and carbon balance (B) of C.
- Fed-Batch bioreactor process of NMeAla production.
- Establishing production of NMeAla from alternative feedstocks.
- Figure 4 Fed-batch cultivation with C.
- Discussion
- Figure 5 Production of NMeAla from alternative carbon sources.
- Experimental
- Bacterial strains and growth conditions.
- Fed-Batch cultivation.
- Molecular genetic techniques and strain construction.
- Table 1 Bacterial trains and vectors used in this study.
- Crude extract preparation and enzyme assays.
- Quantification of amino acids and organic acids.
- Transcriptome analysis using DNA microarrays.
- Acknowledgements