Wittchen, Manuel; Busche, Tobias; Gaspar, Andrew H.; Lee, Ju Huck; Ton-That, Hung; Kalinowski, Jörn; Tauch, Andreas: Transcriptome sequencing of the human pathogen Corynebacterium diphtheriae NCTC 13129 provides detailed insights into its transcriptional landscape and [...]. In: BMC Genomics. Jg.19 H. 1. 2018
Inhalt
- Background
- Methods
- Bacterial strains and culture conditions
- Cell sampling and RNA isolation
- cDNA library preparations and sequencing
- Bioinformatics data analysis
- Results and discussion
- cDNA sequencing and mapping to the C. diphtheriae NCTC 13129 genome
- Identification of transcription start sites of primary transcripts
- Identification of the house-keeping sigma factor σA promoter motif
- Characteristics of 5′-untranslated regions (5’-UTRs)
- 5’-UTR length distributions
- Riboswitches and other RNA motifs
- Ribosomal binding site motif
- Re-annotation of coding sequences and detection of novel transcripts
- Analysis of operon structures by combining the primary 5′-end and the whole transcriptome data sets
- Analysis of the DtxR regulon by comparing two whole transcriptome data sets
- Comprehensive transcriptomic view on the phage island and the tox gene encoding diphtheria toxin
- Conclusion
- Additional files
- Abbreviations
- Funding
- Availability of data and materials
- Authors’ contributions
- Ethics approval and consent to participate
- Consent for publication
- Competing interests
- Publisher’s Note
- Author details
- References