Kamal, Nadia: Comparative analysis of the genetic variation of flowering time genes in Vitis vinifera. 2018
Inhalt
- Summary
- Background
- Vitis vinifera
- Flowering in plant species
- Flowering and flowering time control in grapevine
- Impact of climate change on flowering time
- Flowering time control genes and pathways
- Analysis of the genetic variation of quantitative traits
- Objectives
- Materials and Methods
- Identification and annotation of flowering time control genes
- Flowering time control candidate genes for amplicon sequencing
- Amplimer generation
- Plant material
- The F1-mapping population GF.GA-47-42 x 'Villard Banc'
- Important grapevine cultivars and their flowering time phenotypes
- DNA isolation and amplimer amplification
- Library preparation and amplicon sequencing
- Read processing and mapping
- Allele phasing
- Post-alignment quality enhancement of read alignments
- Variant calling
- Physical phasing of candidate gene alleles
- Determination of allele combinations in F1-individuals
- Association analysis
- Analysis of flowering time control candidate genes in important grapevine cultivars
- Marker development
- Differential gene expression analysis
- Results
- Identification of flowering time control candidate genes in grapevine
- Association analysis of flowering time control-candidate genes and alleles with the timing of flowering
- Primer design and amplimer amplification
- Amplicon sequencing and read processing
- Mapping and variant calling
- Allele Phasing
- Correlation analysis
- Marker development and analysis
- Investigating alleles of FTC candidate genes in grapevine cultivars
- Investigating expression of FTC candidate genes
- Discussion
- Identification of more than four hundred flowering time control candidate genes in grapevine
- Investigating allele combinations of flowering time control candidate genes
- Amplicon sequencing achieves a high level of targeting but suffers from technical difficulties
- Allele phasing was successful in 46 genes of which many exhibit up to four alleles
- Correlation between alleles of candidate genes and flowering time phenotypes in- and outside of QTL-regions
- Correlation values vary in dependence on phenotypic data
- Phenotyping influences genetic association analysis
- E1-alleles of VvTM6 and VvWNK6 correlate with an early flowering phenotype
- Variations in flowering time control candidate genes across cultivars
- Functional allele specific molecular markers were developed
- Differential gene expression analysis of FTC candidate genes indicates their role in the timing of flowering in grapevine
- Conclusion and Outlook
- Acronyms
- List of Figures
- List of Tables
- Bibliography
- Appendix
- Acknowledgments
- Declaration