Diekmann, Robin: Extending the application range of optical nanoscopy. 2017
Inhalt
- Introduction
- Theoretical background
- Widefield fluorescence microscopy
- Approaches to super-resolution fluorescence microscopy
- Single emitter localization accuracy
- Single emitter localization precision
- Resolution estimation in SMLM
- Planar optical waveguides
- Holographic optical trapping
- Camera detectors
- Waveguide chip-based nanoscopy
- Introduction
- Waveguide characterization for near-field excitation in fluorescence microscopy
- Chip-based dSTORM
- Fluctuation-based imaging
- High throughput localization microscopy
- NA-dependent localization precision
- Scaleable super-resolution imaging
- Discussion and outlook
- Nanoscopy of optically trapped samples
- Introduction
- Influence of trap on dSTORM image
- Computational motion compensation
- Imaging optically trapped bacteria in solution
- Multiple perspectives by controlling the sample orientation
- Setup modification for dynamic measurement of protein DNA interaction
- Discussion and outlook
- Cost-efficient solutions for localization microscopy
- Introduction
- Industry-grade CMOS and scientific-grade CMOS performance
- Comparative dSTORM imaging
- High-speed dSTORM imaging
- Detector characterizations
- Compensating for CMOS detector inhomogeneities
- Low-cost nanoscopy of LSECs
- Discussion and outlook
- Comprehensive outlook
- Bibliography
- Materials and Methods
- Acronyms
- Publications
- Acknowledgements