Klein, Benjamin; Wibberg, Daniel; Hallmann, Armin: Whole transcriptome RNA-Seq analysis reveals extensive cell type-specific compartmentalization in Volvox carteri. In: BMC Biology. Jg.15 H. 1. 2017
Inhalt
- Background
- Results
- RNA isolation and high throughput sequencing
- Mapping and analysis of expression data
- Investigation of gene structures
- Gene expression of previously investigated Volvox genes
- Identification of the most highly expressed genes
- Detection of differentially expressed genes between somatic and reproductive cells
- Functional enrichment analysis of the most differentially expressed genes
- Classification of all protein domains and screening for cell type-specific expression
- Discussion
- Conclusion
- Methods
- Strain and culture conditions
- Separation of cell types
- RNA isolation
- Whole transcriptome sequencing
- Mapping, data analysis, and bioinformatics
- Manual examination of gene structures on a random basis
- Annotation
- Additional files
- Abbreviations
- Funding
- Availability of data and materials
- Authors’ contributions
- Ethics approval and consent to participate
- Consent for publication
- Competing interests
- Publisher’s Note
- Author details
- References