Bräutigam, Andrea ; Shrestha, Roshan P.; Whitten, Doug; Wilkerson, Curtis G.; Carr, Kevin M.; Froehlich, John E.; Weber, Andreas P. M.: Low-coverage massively parallel pyrosequencing of cDNAs enables proteomics in non-model species: Comparison of a species-specific database generated by [...]. In: Journal of Biotechnology. Jg.136 H. 1-2. 2008, S. 44-53
Inhalt
- Massively parallel pyrosequencing and generation of sequence databases
- Results
- Properties of the cDNA sequence databases
- Performance of the databases in proteomics
- Abundance of mass spectra is positively correlated with correct protein identification in non-species-specific databases
- Protein hydrophobicity is a poor predictor for correct protein identification
- Analysis of abundant non-identified and of wrongly identified proteins
- Discussion
- Acknowledgements
- Supplementary data
- References