Rosar, Christian; Kanonenberg, Kerstin; Nanda, Arun M.; Mielewczik, Michael; Bräutigam, Andrea ; Novak, Ondrej; Strnad, Miroslav; Walter, Achim; Weber, Andreas P. M.: The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism. In: Molecular Plant. Jg.5 H. 6. 2012, S. 1227-1241
Inhalt
- Plant Growth Rates and Photosynthetic Capacity
- Map-Based Cloning of dov1 and Testing for Allelism
- Mutant dov1 ATase2 Has No Activity In Vitro
- ATASE Activity Is Reduced in dov1 Plants
- Metabolite Profiling of dov1
- Quantitation of Cytokinin Levels and Complementation with Cytokinin
- A Cytokinin Reporter but Not an Auxin Reporter Responds Differentially in dov1
- DISCUSSION
- Dov1 Is Defect in the ATase2 Gene Product
- Consequences of Limited Purine Availability for Primary Leaf Metabolism
- Cytokinins and Altered Growth Patterns of dov1 Leaves
- The Basis of Reticulated Leaf Patterning
- METHODS
- Plant Material, General Growth Conditions, and Plant Lines
- Mapping of dov1
- GUS Staining
- Biochemical Complementation with Cytokinin
- Metabolite Profiling
- Cytokinin Quantification
- Chlorophyll Determination
- Enzymatic Assays
- GROWSCREEN FLUORO Analysis of Growth, Fluorescence, and Phenotypic Properties
- Statistical Analysis
- Alignment of Protein Sequences
- SUPPLEMENTARY DATA
- FUNDING
- ACKNOWLEDGMENTS
- REFERENCES