Ruwe, Matthias; Kalinowski, Jörn; Persicke, Marcus: Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum. In: Frontiers in Microbiology. Jg.8. 2017
Inhalt
- Materials and Methods
- Bacterial Strains and Growth Conditions
- Construction of Strains
- Complementation Test for (p)ppGpp Synthetase Activity in E. coli
- Protein Purification
- (p)ppGpp Synthetase Assays
- Results
- The C. glutamicum Genome Encodes Two Putative Small Alarmone Synthetases (SAS)
- Phylogeny of RelS Homologs
- The Growth Deficit of a C. glutamicum Δrel Strain Is Compensated by the Additional Deletion of relSCg; The Deletion of relPCg Has No Effect
- Expression of RelSCg but Not RelPCg Complements the Multiple Amino Acid Auxotrophy of E. coli ΔrelAΔspoT
- In vitro Analysis of (p)ppGpp Synthetase Activity of RelPCg, RelSCg and RelCg
- RelSCg Shows Unusual Enzyme Kinetics with High Km Values for GTP, GDP, and GMP
- RelSCg Is Highly Mg2+ Dependent and Most Active at Neutral pH Conditions and at Low Temperatures
- Discussion
- Author contributions
- Acknowledgments
- Supplementary Material
- References