Venkanna, Deepak; Südfeld, Christian; Baier, Thomas; Homburg, Sarah Vanessa; Patel, Anant; Wobbe, Lutz; Kruse, Olaf: Knock-Down of the IFR1 Protein Perturbs the Homeostasis of Reactive Electrophile Species and Boosts Photosynthetic Hydrogen Production in <i>Chlamydomonas [...]. In: Frontiers in Plant Science. Jg.8. 2017
Inhalt
- Chemicals
- Strains and Growth Conditions
- Generation of IRL Knock-Down Strains
- Antibody and Recombinant Protein Production
- RNA Extraction and Quantitative Real Time PCR
- SDS-PAGE and Immunoblotting
- Chlorophyll Fluorescence Analyses
- Construction of Fusion Protein and Confocal Microscopy
- Statistical Analysis
- Results
- IFR1 Is an Atypical Short-Chain Dehydrogenase that Accumulates in the Cytosol of C. reinhardtii as a Response to Abiotic Stress
- A Knock-Down of IFR1 Causes Diminished RES-Tolerance
- Prolonged Hydrogen Production by IFR1 Knock-Down Mutants
- Prolonged H2 Production in IFR1 Knock-Down Strains Results from a Sustained PSII Activity
- IFR1 Knock-Down Can Be Applied as a Tool to Further Enhance Hydrogen Production in a Strain with a High Starting Capacity
- Discussion
- Author Contributions
- Funding
- Acknowledgment
- Supplementary Material
- References