Nanda, Arun M.; Heyer, Antonia; Kramer, Christina; Grünberger, Alexander; Kohlheyer, Dietrich; Frunzke, Julia: Analysis of SOS-Induced Spontaneous Prophage Induction in Corynebacterium glutamicum at the Single-Cell Level. In: Journal of Bacteriology. Jg.196 H. 1. 2014, S. 180-188
Inhalt
- MATERIALS AND METHODS
- Bacterial strains, media, and growth conditions.
- Cloning techniques.
- Construction of promoter fusions.
- Fluorescence microscopy.
- Flow cytometry.
- Statistical analysis.
- Microfluidic cultivation.
- RESULTS
- The prophage CGP3 is spontaneously induced in single cells.
- Spontaneous PrecA induction in single cells.
- Spontaneous PrecA activity reflects a bona fide SOS response.
- Time-lapse analysis of PrecA activation dynamics.
- Correlating prophage activity to an induced SOS response in single cells.
- Impact of growth phase on spontaneous SOS and prophage activity.
- DISCUSSION
- ACKNOWLEDGMENTS
- REFERENCES