Taniguchi, Hironori; Busche, Tobias; Patschkowski, Thomas; Niehaus, Karsten; Patek, Miroslav; Kalinowski, Jörn; Wendisch, Volker F.: Physiological roles of sigma factor SigD in Corynebacterium glutamicum. In: BMC Microbiology. Jg.17 H. 1. 2017
Inhalt
- Methods
- Bacterial strains, plasmids and oligonucleotides
- Medium and conditions for growth experiments
- Photometric determination of supernatant turbidity and observation of cell aggregation by microscopy
- Protein analysis of the supernatant
- Quantification of carbohydrate in acetone precipitates
- RNA extraction
- RNA isolation, library preparation and RNA-seq
- Read mapping, data visualization and analysis of gene expression
- Real-time PCR analysis
- Detection of trehalose dicorynomycolate by thin layer chromatography
- Results
- Deletion and overexpression of sigD influenced the maximum growth rate
- sigD overexpression influenced cell culture characteristics
- sigD overexpression changed the pattern of secreted proteins
- sigD overexpression induced the secretion of carbohydrate-containing compounds
- SigD regulated transcription of several genes related to cell envelope integrity
- Overexpression of sigD increased the amounts of trehalose dicorynomycolate
- Discussion
- Conclusion
- Additional files
- Abbreviations
- Funding
- Availability of data and materials
- Authors’ contributions
- Author’s information
- Ethics approval and consent to participate
- Consent for publication
- Competing interests
- Publisher’s Note
- Author details
- References