Taniguchi, Hironori; Henke, Nadja Alina; Heider, Sabine; Wendisch, Volker F.: Overexpression of the primary sigma factor gene sigA improved carotenoid production by Corynebacterium glutamicum: application to production of beta-carotene [...]. In: Metabolic Engineering Communications. Jg.4. 2017, S. 1-11
Inhalt
- Bacterial strains, plasmids and oligonucleotides
- Chromosomal deletion of sigB in C. glutamicum
- Medium, growth condition and growth rate comparison
- Carotenoid extraction and quantification
- Transcriptome analysis of the sigA overexpressing strain using DNA microarray
- Results
- Overexpression of sigA improved production of lycopene by C. glutamicum
- C. glutamicum wild type overproduced decaprenoxanthin in the stationary growth phase as consequence of sigA overexpression
- Application of sigA overexpression to production of the non-native cyclic C40 carotenoid β-carotene
- Engineering of C. glutamicum for overproduction of a non-native linear C50 carotenoid
- Global gene expression changes due to sigA overexpression
- Deletion of the sigma factor gene sigB increased carotenoid production
- Discussion
- Conclusion
- Acknowledgements
- Supporting information
- References