Schwarzhans, Jan Philipp; Wibberg, Daniel; Winkler, Anika; Luttermann, Tobias; Kalinowski, Jörn; Friehs, Karl: Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing. In: Scientific Reports. Jg.6 H. 1. 2016
Inhalt
- Identification of clones with abnormal colony morphology.
- Figure 1. Colony morphology of wildtype P.
- Table 1. Characteristics of P.
- Figure 2. The different kinds of integration events discovered in sequenced P.
- Table 2. Primers used for PCR assays and qRT-PCR.
- Figure 3. Proposed mechanism facilitating the relocation of the AOX1 locus as found in clone JPS014.
- Figure 4. Observed modification of E.
- Discussion
- Figure 5. Relative transcriptional levels of E.
- Methods
- Figure 6. Schematic depiction of the proposed mechanism facilitating integration of E.
- Identification of Mut-phenotype and colony morphology.
- Isolation of genomic DNA and PCR assays.
- Genome sequencing and bioinformatic analysis of P. pastoris strains.
- Genome sequencing, annotation and sequence comparison of the E. coli KRX genome.
- qRT-PCR experiments.
- Acknowledgements
- Author Contributions