Mönkemöller, Viola: Optical super-resolution microscopy of the structure and dynamics of cellular nanopores. 2016
Inhalt
- Introduction
- Optical nanoscopy
- Microscopy techniques
- Fluorescence microscopy
- Photon-reassignment microscopy
- Optical super-resolution microscopy
- LSECs - Liver Sinusoidal Endothelial Cells
- Function and morphology
- Human LSECs in 3D-structured illumination microscopy
- Preparation of LSECs from rat
- Freezing and thawing of LSECs
- Confocal microscopy images of LSECs
- LSECs imaged by SMLM compared to 3D-SIM
- dSTORM on LSECs
- Evaluation of the fenestration diameter in dSTORM vs. 3D-SIM
- Acquisition speed
- Conclusion
- LSECs imaged by SMLM and 3D-SIM on the same setup
- Correlative SMLM and 3D-SIM on the same setup
- Correlative SMLM and 3D-SIM of LSECs
- Rapid multi-color 3D-SIM overviews combined with super-resolution of SMLM
- Correlation between the membrane and cytoskeleton in LSECs
- Fluorescence stains and their effect on living cells
- Fenestration dynamics and structural changes of living LSECs
- Fenestration dynamics of living LSECs
- Effect of different substances on living rat LSECs
- Ethanol and paracetamol treatment
- Instant SIM and its extension to two-photon microscopy
- Further super-resolution projects
- Visualizing lipid nanodomains in mammalian cells
- Open-source image reconstruction of super-resolution SIM data in ImageJ
- Nanoparticles as nonfluorescent analogues of fluorophores
- Conclusions and outlook
- Preparation and staining protocols
- LSECs - Liver Sinusoidal Endothelial Cells
- LSECs imaged by SMLM compared to 3D-SIM
- LSECs imaged by SMLM and 3D-SIM on the same setup
- Fluorescence stains and their effect on live cells
- Fenestration dynamics and structural changes of living LSECs
- Instant SIM and its extension to two-photon microscopy
- Visualizing lipid nanodomains in mammalian cells
- Open-source image reconstruction of super-resolution SIM data in ImageJ
- Nanoparticles as nonfluorescent analogues of fluorophores
- Materials and methods
- Acknowledgment