Matano, Christian; Kolkenbrock, Stephan; Hamer, Stefanie N.; Sgobba, Elvira; Moerschbacher, Bruno M.; Wendisch, Volker F.: Corynebacterium glutamicum possesses β-N-acetylglucosaminidase. In: BMC Microbiology. Jg.16 H. 1. 2016
Inhalt
- Results
- C. glutamicum ATCC13032 possesses a GH3 N-acetylglucosaminidase
- Overexpression of nagA2 fused with homologous N-terminal signal peptides increased extracellular activity of the enzyme
- Overexpression of ChiB fused with a secretion signal peptide from C. glutamicum enabled secretion of the enzyme
- Concomitant overproduction of ChiB and NagA2 is not sufficient to support growth with colloidal chitin in a C. glutamicum strain able to grow with GlcNAc
- Discussion
- Conclusion
- Methods
- Microorganisms, media, and cultivation conditions
- Preparation of colloidal chitin
- DNA preparation, manipulation and transformation
- Construction of plasmids and strains
- Glycol chitin preparation and dot blot chitinase assay
- Chitinase and N-acetylglucosaminidase activity assays
- Quantification of GlcNAc via DNS colorimetric assay
- Additional file
- Acknowledgements
- Funding
- Availability of data and material
- Authors’ contributions
- Competing interests
- Consent for publication
- Ethics approval and consent to participate
- Author details
- References