Henke, Nadja Alina; Heider, Sabine; Peters-Wendisch, Petra; Wendisch, Volker F.: Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum. In: Marine Drugs. Jg.14 H. 7. 2016
Inhalt
- Results
- Construction of a -Carotene Producing C. glutamicum Base Strain
- Design of the Combinatorial Gene Assembly and Library Construction for Engineering Astaxanthin Production in C. glutamicum
- Combinatorial Engineering Covered Vastly Different Astaxanthin, -Carotene, Zeaxanthin and Canthaxanthin Titers
- In Silico Analysis of the Carotenogenic Genes crtZ and crtW from Marine and Non-Marine Bacteria for Heterologous Expression in C. glutamicum
- High Astaxanthin Production by C. glutamicum Strains Expressing crtW and crtZ from F. pelagi
- Discussion
- Materials and Methods
- Bacterial Strains, Media and Growth Conditions
- Recombinant DNA Work
- Construction of Expression Vector pSH1
- Deletion and Exchenge Mutagenesis in the Genome of C. glutamicum
- Combinatorial Gene Assembly, Library Construction and Overexpression of Carotenogenic Genes
- Extraction and Quantification of Carotenoids