Hofemeier, Arne D.; Hachmeister, Henning; Pilger, Christian; Schürmann, Matthias; Greiner, Johannes; Nolte, Lena; Sudhoff, Holger; Kaltschmidt, Christian; Huser, Thomas; Kaltschmidt, Barbara: Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells. In: Scientific Reports. Jg.6 H. 1. 2016
Inhalt
- Figure 1. Raman spectroscopy successfully shows calcium deposition occurring during osteogenic in vitro differentiation of nasal stem cells.
- Discussion
- Figure 2. Characteristic expression of Osteocalcin validates successful osteogenic differentiation of ITSCs.
- Conclusions
- Figure 3. Successful imaging of hydroxyapatite and collagen in human skull bone using CARS and SHG microscopy.
- Figure 4. ITSCs cultivated under control conditions show no signs of calcium hydroxyapatite deposition or collagen detectable with CARS and SHG imaging.
- Figure 5. CARS and SHG imaging reveal calcium hydroxyapatite deposits and collagen after osteogenic differentiation of ITSCs.
- Figure 6. CARS and SHG microscopy reveal three-dimensionality of ITSC-derived calcium hydroxyapatite deposits embedded in collagen.
- Materials and Methods
- Human material.
- Mouse material.
- Isolation and culturing of inferior turbinate stem cells (ITSCs).
- Osteogenic differentiation of ITSCs.
- Reverse transcription PCR.
- Raman spectroscopy.
- Histochemical staining (Alizarin Red Staining).
- CARS and SHG microscopy.
- Processing of CARS and SHG images.
- Acknowledgements
- Author Contributions