Steffens, Tim; Vorhölter, Frank-Jörg; Giampa, Marco; Hublik, Gerd; Pühler, Alfred; Niehaus, Karsten: The influence of a modified lipopolysaccharide O-antigen on the biosynthesis of xanthan in Xanthomonas campestris pv. campestris B100. In: BMC Microbiology. Jg.16 H. 1. 2016
Inhalt
- Background
- Results and discussion
- The genetic background of three X. campestris pv. campestris B100 strains carrying mutated genes involved in the biosynthesis of the LPS O-antigen
- Biochemical analysis of three X. campestris pv. campestris B100 mutant strains revealed a missing O-antigen for Xcc H21012 (wxcB) and a modified O-antigen for Xcc H20110 (wxcN) and Xcc H28110 (wxcK)
- Xanthan production of X. campestris pv. campestris B100 mutants characterized by a missing or modified O-antigen
- The influence of a missing or a modified LPS O-antigen on the xanthan biosynthesis in X. campestris pv. campestris B100
- Conclusions
- Methods
- Strains and cultivation
- Glucose determination
- Nitrate determination
- Xanthan and biomass determination
- LPS isolation
- Methanolysis and peracetylation of LPS
- Hydrolysis, reduction and peracetylation of LPS
- Gas Chromatography (GC) (Mass Spectrometry)
- Sample preparation for MALDI-MS analysis
- MALDI measurements
- Additional files
- Abbreviations
- Acknowledgments
- Funding
- Availability of data
- Authors’ contributions
- Competing interests
- Consent for publication
- Ethics approval and consent to participate
- Author details
- References