Schwarzhans, Jan Philipp; Wibberg, Daniel; Winkler, Anika; Luttermann, Tobias; Kalinowski, Jörn; Friehs, Karl: Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector opti [...]. In: Microbial Cell Factories. Jg.15 H. 1. 2016
Inhalt
- Characterization and grouping of pAHBgl-GFP P. pastoris clones
- Selection of clones for sequencing and general sequencing results
- Correlation between sequencing and experimental results
- High producers
- Over- and underachievers
- Low-non-producers
- An optimized vector to prevent false-positive clones
- Conclusion
- Methods
- Microorganisms and cultivation conditions
- Plasmid construction and transformation
- Characterization of P. pastoris clones
- Sequencing of 31 selected P. pastoris strains and genome assembly
- Bioinformatic analyses of the 31 Pichia pastoris strains
- Authors’ contributions
- References