Pauli, Martina; Chakarov, Nayden; Rupp, Oliver; Kalinowski, Jörn; Goesmann, Alexander; Sorenson, Michael; Krüger, Oliver; Hoffman, Joseph: De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite. In: BMC Genomics. Jg.16 H. 1. 2015
Inhalt
- Methods
- Sampling
- RNA isolation, cDNA generation and sequencing
- Quality filtering
- Sequence assembly
- BLAST mapping, sequence annotation and comparative genomics
- Mining for molecular markers
- Assigning transcripts to the four pools
- Transcripts found in different tissues
- qPCR analysis
- Results
- Total RNA yields
- Sequence data and transcriptome assembly
- Estimating data loss
- Functional annotation
- Molecular marker discovery
- Differential expression patterns and melanin pathway characterisation
- qPCR analysis
- Discussion
- Assembly and functional annotation
- Parasite transcripts
- Candidate genes for fitness differences
- qPCR analysis
- Conclusion
- Additional file
- Competing interests
- Authors’ contribution
- Acknowledgements
- Author details
- References