Leßmeier, Lennart; Wendisch, Volker F.: Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum. In: BMC Microbiology. Jg.15 H. 1. 2015
Inhalt
- Results
- Biphasic, non-linear growth response of C. glutamicum wild type to methanol
- Adaptive laboratory evolution of C. glutamicum in the presence of methanol
- Role of the linear methanol detoxification pathway
- DNA microarray analysis of global gene expression of C. glutamicum Tol1
- Genome sequencing of Tol1 and introduction of mutations into the wild-type genome
- Influence of mutations increasing methanol tolerance on growth with ethanol
- Discussion
- Conclusions
- Methods
- Microorganisms and cultivation conditions
- Adaptive laboratory evolution
- DNA preparation, manipulation and transformation
- Construction of C. glutamicum mutant strains
- Alcohol dehydrogenase enzyme assays
- Analysis of formaldehyde degradation
- Transcriptome analysis using DNA microarrays
- Genome sequence analysis
- Availability of supporting data
- Additional file
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgements
- References