Freudenau, Inga; Lutter, Petra; Baier, Ruth; Schleef, Martin; Bednarz, Hanna; Lara, Alvaro R.; Niehaus, Karsten: ColE1-Plasmid Production in Escherichia coli: Mathematical Simulation and Experimental Validation. In: Frontiers in Bioengineering and Biotechnology. Jg.3. 2015
Inhalt
- Materials and Methods
- Bacterial Strain and Plasmids
- Cultivation
- Growth Rate Determination
- Determination of the RNAI and RNAII Concentrations
- Determination of the Plasmid DNA Concentrations
- The Theoretical Model
- The Dynamic Model
- Results
- Cultivation and Determination of the Growth Rate for E. coli DH5α Carrying a Low or a High Copy Plasmid
- Determination of the RNAI/RNAII and the pDNA Concentrations
- The Structural Model of ColE1-like Plasmid Replication Control
- The Dynamic Model of ColE1-like Plasmid Replication Control
- Simulations of the ColE1-like Plasmid Replication Control – Simulations for Parameter Fitting
- Predictive Simulations
- Discussion
- Conclusion
- Acknowledgments
- Supplementary Material
- References