Taniguchi, Hironori; Wendisch, Volker F.: Exploring the role of sigma factor gene expression on production by Corynebacterium glutamicum: sigma factor H and FMN as example. In: Frontiers in Microbiology. Jg.6. 2015
Inhalt
- Bacterial Strains, Plasmid, and Primer
- Medium and Growth Condition
- Riboflavin Production Experiments
- Transcriptome Analysis of sigH Overexpressing Strain using DNA Microarrays
- Measurement of Glucose-6-Phosphate 1-Dehydrogenase Enzyme Activities
- FMN and FAD Production Experiments
- Results
- Effect of Overexpressing Sigma Factor Genes in C. glutamicum
- Overexpression of sigH Resulted in Riboflavin Secretion
- Global Gene Expression Changes due to sigH Overexpression
- FMN Production by C. glutamicum Established as Proof-of-Concept based on Overexpression of Endogenous Genes sigH and ribF
- Discussion
- Acknowledgments
- References