Klatte, Stephanie; Wendisch, Volker F.: Role of L-alanine for redox self-sufficient amination of alcohols. In: Microbial Cell Factories. Jg.14 H. 1. 2015
Inhalt
- Background
- Results
- Construction of the whole cell biocatalyst W3110/pTrc99A-ald-adh-taCv and its comparison to W3110/pTrc99A-ald-adh-taVf invitro and invivo
- Influence of the reaction temperature on amination of 1,10-decanediol to 1,10-diaminodecane by W3110/pTrc99a-ald-adh-taVf and W3110/pTrc99a-ald-adh-taCv
- The role of L-alanine for the redox self-sufficient amination of alcohols in a whole cell process
- Discussion
- Conclusions
- Materials and method
- Bacterial strains, plasmids and oligonucleotides
- Cultivation conditions and media
- Preparation of cell free extract and enzyme assay
- Measurement of alanine dehydrogenase activity
- Measurement of alcohol dehydrogenase activity
- Measurement of transaminase activity
- Whole cell biotransformation with resting cells
- HPLC-analysis
- Competing interests
- Authors’ contribution
- Acknowledgement
- References