Huep, Gunnar; Kleinbölting, Nils; Weisshaar, Bernd: An easy-to-use primer design tool to address paralogous loci and T-DNA insertion sites in the genome of Arabidopsis thaliana. In: Plant Methods. Jg.10 H. 1. 2014
Inhalt
- Results and discussion
- FSTs and T-DNA insertion site predictions
- Primers in paralogous regions of the genome
- Application of the paralog primer method
- Access to the easy-to-use primer design tool
- Conclusions
- Methods
- Terminology
- Categorisation of insertion sites
- Definition of paralog groups
- Primer design avoiding multiple annealing sites for the 3′-end
- Primer3-based method
- Paralog primer method
- Additional file
- Competing interests
- Authors’ contributions
- Authors’ information
- Acknowledgements
- References