Breitsprecher, Dennis; Gherardi, Ermanno; Bleymüller, Willem; Niemann, Hartmut: Crystal structure of an engineered YopM-InlB hybrid protein. In: BMC Structural Biology. Jg.14 H. 1. 2014
Inhalt
- Results
- Generation and biochemical characterization of YopM-InlB
- Structure of YopM-InlB
- YopM-InlB has reduced affinity for MET
- YopM-InlB is at least 10-fold less active in MET phosphorylation than wild type InlB321
- A YopM-InlB hybrid including the InlB C-terminal domains induces cell scatter though less efficiently than wild type InlB
- Discussion
- The internalin domain is a versatile framework allowing targeted manipulations
- The InlB cap domain is not essential for MET activation
- Outlook
- Conclusions
- Methods
- Cloning of YopM-InlB
- Protein expression and purification
- Crystallization, data collection and structure determination
- Differential scanning fluorimetry
- Analytical gel filtration
- Binding, phosphorylation and cell scatter assays
- Competing interests
- Authors’ contributions
- Acknowledgement
- Author details
- References