Komati Reddy, Gajendar; Wendisch, Volker F.: Characterization of 3-phosphoglycerate kinase from Corynebacterium glutamicum and its impact on amino acid production. In: BMC Microbiology. Jg.14 H. 1. 2014
Inhalt
- Results
- Phylogenetic analysis of PGK from C. glutamicum
- Purification of PGK of C. glutamicum from recombinant E. coli expressing cg1790/pgk
- Characterization of PGK from C. glutamicum
- Kinetic parameters of PGK from C. glutamicum
- Physiological studies of pgk deletion mutants
- Effect of pgk overexpression on amino acid production
- Discussion
- Conclusions
- Methods
- Microorganisms and cultivation conditions
- Overexpression of pgk in C. glutamicum
- Overproduction of PGK in E. coli, protein purification and molecular weight determination
- Assay conditions
- Overexpression of pgk in production strains
- Computational analysis
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgements
- References