Kong, Xiang Yi; Nesset, Cecilie K.; Damme, Markus; Loeberg, Else-Marit; Lübke, Torben; Maehlen, Jan; Andersson, Kristin B.; Roos, Norbert; Thoresen, G. Hege; Rustan, Arild C.; Kase, Eili T.; Eskild, Winnie: Loss of lysosomal membrane protein NCU-G1 in mice results in spontaneous liver fibrosis with accumulation of lipofuscin and iron in Kupffer cells. In: Disease Models & Mechanisms. Jg.7 H. 3. 2014, S. 351-362
Inhalt
- Phenotype of Ncu-g1gt/gt mice
- Inflammation and excess collagen in Ncu-g1gt/gt liver
- Increased rate of cell death and oxidative stress in Ncu-g1gt/gt
- Active fibrogenesis in Ncu-g1gt/gt liver
- Fig.€1. Verification
- Fig.€2. Loss
- Accumulation of autofluorescent material in Ncu-g1gt/gt Kupffer cells
- Fig.€3. Inflammation,
- Fig.€4. Increased
- Fig.€5. Activated
- Fig.€6. Intralysosomal
- Analysis of gene expression
- Fig.€7. Reduced
- Histochemistry
- Immunohistochemistry
- Immunofluorescence
- Transmission electron microscopy (TEM)
- Sirius-red quantification of total liver collagen
- Western blotting
- Enzyme activity assays
- Serum analysis
- Statistical methods