Baumann, Tobias; Arndt, Katja M; Müller, Kristian: Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V. In: BMC Biotechnology. Jg.13 H. 1. 2013
Inhalt
- Results and discussion
- Non-directional ampicillin resistance cassette cloning
- Directional cloning of a RFP reporter device
- Directional cloning of an eukaryotic coding sequence
- High fidelity cloning
- Conclusions
- Methods
- Plasmids and strains
- Design of deoxyinosine-containing primers
- PCR-based amplification of target DNA fragments
- Endonuclease V treatment
- Ligation with plasmid vector fragments
- Analysis of transformants
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgements
- Author details
- References