Müller, Sara; Galliardt, Helena; Schneider, Jessica; Barisas, B. George; Seidel, Thorsten: Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells. In: Frontiers in Plant Science. Jg.4. 2013
Inhalt
- Application of Fret in Living Cells
- Measuring Fret in Living Plant Cells
- Methods For Fret-Analysis—An Overview
- Acceptor Bleaching
- Donor Bleaching
- Fluorescence Lifetime of the Donor
- Spectral Imaging
- Detecting Sensitized Emission by Ratio-Imaging
- 3-Filter FRET (Sensitized Emission)
- Calibration and Quantification Procedures for Filter FRET
- Conclusions and Future Perspectives
- Materials and Methods
- Isolation and Transfection of Protoplasts
- Heterologous Expression of Fluorescent Proteins
- Spectral Analysis of Recombinant Fluorescent Proteins
- Calculation of R0
- FRET Measurements
- Acknowledgments
- References