Vold Korgaard Jensen, Jaide; Wendisch, Volker F.: Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum. In: Microbial Cell Factories. Jg.12 H. 1. 2013
Inhalt
- Results
- Proline utilisation
- Plasmid-based overexpression of ocd
- Identification of key medium components for proline production
- Coupling growth to proline production
- Discussion
- Conclusions
- Methods
- Strains, plasmids, and media
- Culture conditions
- Utilisation of proline as carbon and nitrogen source
- Screening of medium components using the Plackett-Burman design
- Ornithine cyclodeaminase assay
- N-acetylglutamate kinase assay
- Amino acid and glucose determination
- Competing interests
- Authors’ contributions
- Acknowledgements
- References